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We specifically chose HOXD3 as it represents an interesting class of genes that appear to show a pattern of increased methylation correlating with tumour grade progression according to the classic Gleason pattern grading system within Gleason score 6 and 8 tumours.
Three white spotting cat phenotypes were assigned according to the white pattern grading system proposed by Robinson (1959): solid, bicoloured and van (Fig. 1).
Pattern grading, making sets of patterns to fit a range of sizes, is the next step in the design process.
Formerly pattern grading was a completely manual drafting process, but in the 1950s pattern-grade machines were invented to increase the speed of grade-drafting patterns.
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21 painDETECT is a nine-item questionnaire that consists of seven sensory symptom items for pain that are graded from 0= never to 5= strongly, one temporal item on pain-course pattern graded −1 to +1, and one spatial item on pain radiation graded 0 for no radiation or +2 for radiating pain.
The Valsalva manoeuvre is also very helpful in distinguishing a reversible restrictive pattern (grade III) from an irreversible (grade IV) one, because with this manoeuvre the reversible restrictive pattern can turn into an abnormal relaxation pattern, whereas the irreversible restrictive pattern remains unchanged.
The pathologic factors analyzed included lymphovascular invasion, invasive tumor pattern, grade of differentiation, mucin production, and intratumoral lymphocyte infiltration.
Additionally all members of groups B and D exhibited the impaired relaxation pattern (grade 1 LVDD) in the echocardiograms.
All the animals exhibited a normal locomotion pattern (grade 0), and neurological deficits were not detected, according to the classification of Mayhew et al. [ 11].
Three-tiered grading was used for endometrioid adenocarcinomas, based on the FIGO recommendations for nonsquamous architectural grading where grade 1 (G1) has ⩽5% solid growth pattern, grade 2 (G2) has between 6 and 50% solid growth pattern, and grade 3 (G3) has >50% solid growth pattern.
The histological grade was determined according to the criteria of Szanto et al.: Grade 1, tumor with tubular and cribriform pattern; Grade 2, tumor with a pure cribriform pattern or mixed with less than 30% solid areas; and Grade 3, tumor with more than 30% solid areas [ 3].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com