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These data suggest that there is a temporal pattern of replication for some functionally related genes.
At the same time, the cells of the cancer-free urology patients exhibited the normal pattern of replication: high SD values for SNRPN and significantly lower 10-122) values for CEN15 (Fig. 2a).
G-code can be compiled to automatically step out a full wafer of pattern for rapid replication or scaling of the original stamp pattern (an example of which may be found in the Additional file 1).
Each of the non-cancer patients showed the expected synchronous patterns of replication for each of these loci.
The GOstat results in Table 5 give us an indication that there are temporal patterns of replication for some functionally related genes.
New phenotypes were tested in the same cohorts and new cohorts were tested for the same associations, each having somewhat limited success, resulting in a complicated pattern of replication and non-replication that hinders final interpretation [ 11, 36 49].
We next asked whether there are associated patterns of replication timing alterations in other cancer cell types.
In addition, we also studied the replication pattern for the gene coding for the amyloid precursor protein (APP), which we have recently shown to be monoallelically expressed [15].
As represented in the three panels of Figure 3A, we can observe a pattern of viral replication throughout time for all different shRNA clones.
DNMT1 has a substrate preference for hemimethylated DNA, maintaining methylation patterns during replication, 41 whereas DNMT3A/B methylates DNA de novo, irrespective of any methylation.
The inactivated origins did not globally alter the pattern of genome replication, allowing investigation of the consequences for termination.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com