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Visual flow pattern experiments were conducted in a vacuum atmosphere.
As a tentative solution, we secondly present experimental methods to assess bank strength effects that can be used in the design of river pattern experiments.
The candidate P450s and UGTs that may participate in SS biosynthesis were screened via methyl jasmonate (MeJA) inducibility and tissue-specific expression pattern experiments.
For the tissue-specific expression pattern experiments, five tissues (roots, stems, leaves, flowers, and fruits) were collected and similarly restored as described in our previous report [ 81].
MeJA inducibility and tissue-specific expression pattern experiments were used to screen two P450s and three UGTs that may be involved in SS biosynthesis.
Through MJ-inducible expression pattern experiments and GC-MS analysis, we found that their expression was significantly induced by MJ, and sesquiterpenes content was elevated accordingly.
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We consider that the low growth in the growth pattern experiment was affected by desiccation.
Even during the culture duration (7 days), terminal filament lengths were shorter in the growth pattern experiment, in which we performed daily length measurements compared with those in the SGR experiment, which was static cultured.
For the tissue-specific expression pattern experiment, β-tubulin was selected as the internal reference gene based on our previous research [ 81].
STRING can predict these interactions based on neighbourhood, gene fusion products, homology and similarity of coexpression patterning, experiments, databases and textmining.
Moreover, the expression patterns experiments of synthetic genes will generate an improved understanding of their functional characteristics.
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