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pHH3+insulin+ (with punctuated pattern) cells were counted as β-cells at G2 phase.
Controlled 3D Cell Pattern: Cells were patterned on the 3D matrices into discrete zones.
The percentage of mostly nuclear (N > C), even distribution (N = C), or mostly cytoplasmic (N < C) of Smurf2GFP pattern cells was calculated based images of 40 cells at each data point.
Without showing any systematic pattern, cells were seen to actively weave their way through the ordered network of immobile Sertoli cells, over a range of approximately 20 150 μm within a single day.
Both the test pattern and H-pattern cells are analyzed using intensity-dependent photoluminescence imaging (Suns-PL), and the H-pattern cells are additionally probed at each busbar to monitor their open-circuit voltage (via Suns-Voc measurements).
During growth and patterning, cells are specified by both quantitative and directional information.
Unexpectedly, CTCF binding patterns in normal cells were remarkably different from those in immortal cells.
Gleason pattern 3 cells are thought to represent the disease at an early, well-differentiated, stage.
Both Th1 and Th2 cytokine pattern producing cells are linked with bone resorption.
These cycles are self-sustaining oscillatory patterns: once cells are synchronized, they continue to exhibit robust oscillations indefinitely [ 3, 4].
In all pattern widths, cells were highly aligned, with maximum cell and nuclear elongation occurring for the 10 μm pattern width.
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