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Strikingly, latrunculin A treatment of Podocalyxin-transfected cells led to loss of actin filaments, including apically localized f-actin, and redistribution of Podocalyxin into a diffuse pattern at the cell surface.
Similarly, LAMP1 staining in hypertrophic chondrocytes of the growth plate, close to the bony interface, was detected in a punctate pattern at the cell surface (Fig. 1C arrows).
Given the differences in sialylation between LNCaP and C4-2B cellsuggestinging an altered sialylated glycol epitope pattern at the cell surface of C4-2B cells, we studied whether these changes in sialylation influence the adhesion of LNCaP and C4-2B cells to collagen type I.
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While some atrial myocytes show gap junctions organized in classic intercalated disc patterns at the cell ends, others show 'spread' of the discs along the lateral aspects of the cell.
Confocal microscopic analysis revealed colocalization of f-actin and Podocalyxin in a punctate pattern at the apical cell surface where microvilli form in untreated Podocalyxin-transfected cells (figure 2).
For example, cell surface expression levels of MHC class I have been shown to determine both the protein pattern at the NK cell immune synapse and subsequent cytotoxicity [38].
Immunostaining human embryos for H3-K4me3 also revealed a differential histone modification expression pattern at the eight-cell stage of human development in addition to positive signals for H3-K4me3 and H3-S28P in one of the pronuclei and polar bodies at the one-cell stage, respectively (Fig. 3C and Supplementary Material, Fig. S3b).
The S1/S2 segments of the proximal tubule exhibit a diffuse pattern of labeling along the basolateral cell surface and a fine-thread-like pattern of labeling at the cell contacts near the apical cell surface.
A similar pattern of attachment at the cell junction has been seen in vivo, and it has been suggested that this distribution may facilitate penetration of the epithelial layer [28].
As presented in Fig. 2, Cx32 was regularly found with the typical punctuated pattern of distribution at the cell membrane in small and larger clusters of transplanted normal hepatocytes (Fig. 2a c, g).
In contrast, Fry showed no obvious pattern at the basal follicle cell surface (data not shown).
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