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The Memorial of the title is the Memorial Medical Centre where, for five days, staff and patients including a unit – run by a separate company – for terminally ill patients were cut off by the rising waters and by a city plunged into looting.
Hundreds of patients were cut open and scarred, prosecutors said, and some needed corrective surgery.
Paraffin-embedded samples from HT patients were cut into 3 μm thick sections.
Biopsies from patients were cut in small pieces (1-2 mm) and fixed in McDowell's fixative as previously described [ 29].
Freshly resected SEGA samples from two patients were cut into small pieces and trypsynized for 1 h at 37 °C.
Brain metastases from four patients were cut into 4-micron sections and stained by dual colorimetric IHC (Envision G/2 Doublestain; Dako) for PR (NeoMarkers, Freemont, CA, USA) and CK5 (Novocastra, Leica Biosystems, Nussloch, Germany) [ 22].
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In brief, the mucosa from surgical patients was cut into 1-mm pieces and epithelial cells were removed in Hank's Balanced Salt Solution (HBSS) without Ca2+ and Mg2+ (PAA, Cölbe, Germany) with 2mM ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich, Taufkirchen, Germany).
Tissue from patients was cut in 2 5 μm thick sections.
Paraffin-embedded tumour material of 178 patients was cut into 4 μm-thick sections and placed onto glass slides.
Formalin-fixed paraffin-embedded tissue blocks from each patient were cut at 4-μm thickness.
Serial sections from at least six different biopsy samples per patient were cut with a cryostat (5 μm) and fixed with acetone, and the endogenous peroxidase activity was blocked with 0.3% hydrogen peroxide in 0.1% sodium azide/PBS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com