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performed primary cell and patient specimen studies.
Methods: Three different hospitals participated in a patient specimen and analytical validation study (n=186) for the i-STAT cTnI assay carried out in real time.
This is the first systematic review of the effectiveness of barcoding practices for reducing patient specimen and laboratory testing identification errors.
Upon receipt of a completed Tumor Profiling Requisition form, the laboratory will coordinate with the Surgical Pathology or Cytopathology division of the Department of Pathology to obtain the appropriate patient specimen(s), whether from within the medical center or from an outside laboratory.
All subjects provided written informed consent and The Indiana University-Clarian Institutional Review Board IRBB) approved all procedures for patient specimen collection and data handling.
Three patients each underwent surgical procedures on two occasions from which we obtained tissue: Specimen #M2 and M7 were from the same patient; specimen #M30 and M33 were from the same patient; specimen #M38 and M41 were from the same patient.
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Heterogeneity occurs at two levels: as multiple phenotypes within an individual (patient-specimen heterogeneity) and between individuals (disease heterogeneity).
Both systems provide web-based data entry, support of cross-validation and plausibility checks, a (logical) central database, an elaborated security concept with multi-tier pseudonymity for patient-, specimen- and image-identifiers.
For LCD, lymph node sections were stained with haematoxylin and eosin (H&E) staining kit (Molecular Machines Industries, Glattbrugg, Switzerland), and lymphoma cells (1,000 cells/patient specimen) were isolated using the MMI CellCut (MMI).
In genotyping multiple lesions of different stage from the same patient/specimen, we found considerable heterogeneity; most notably, in 12 specimens the proliferative lesion was PIK3CA mutant but the concurrent carcinoma was wild type.
For this reason patient/specimen allocation will remain blinded.
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CEO of Professional Science Editing for Scientists @ prosciediting.com