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Thus, to understand the molecular mechanisms in hypoxia, more attention should be paid to the analysis of these pathways, which contain many hypoxia-regulated proteins.
Positive Z-scores > 0 indicated pathways which contain significantly regulated genes from the DNA microarray data sets in an overrepresentative manner [ 32].
In the signaling pathways diagram, a simplistic representation of the interactions between the measured phosphoproteins, the pathways which contain those proteins, and the effect of the targeted inhibitors can be observed.
In addition, the apoptosis, Wnt signaling, and C21-Steroid hormone metabolism pathways, which contain target genes of the let-7 family, are important for granulosa cell proliferation, follicular growth and development, and luteinic formation and regression.
The illustrations also demonstrate the usefulness of an integrative analysis to discover disrupted pathways, which contain many member genes each having low significance with respect to copy number changes.
After the filtering, the SBML file contains : 553 compounds and 597 reactions (the nodes of the network represented in Metaviz) 198 metabolic pathways of which 30 are superpathways, i.e. pathways which contain other pathways.
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The top KEGG pathways which contained the highest number of DEGs in leaves and roots were metabolic pathways, biosynthesis of secondary metabolites, plant hormone signal transduction, ribosome and RNA transport.
GSEA was run on each data set separately, excluding pathways which contained less than 12 genes or more than 400 genes, to improve specificity and then significance of the obtained Enrichment Score was assessed by performing 1000 random permutations on the data.
Using this program, it was possible to create protein networks and pathways, which contained the proteins displaying changes in intensity and thereby placing individual protein data into physiological context.
Negative regulation of miR166 expression through the SHL/SHO pathway, which contains orthologs of Arabidopsis proteins implicated in ta-siRNA generation [6, 7, 10, 11], suggest that there is a link between RNA-mediated gene regulation and fundamental plant processes such as embryonic SAM formation.
The PLP-dependent trans-sulfuration pathway, which contains both CBS and CSE for H2S production, is localized in the cytosol.
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