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The putative genes encoding the factors involved in light and circadian signaling pathways were successfully isolated in P. bellina transcriptome, and several ones showed higher expression, including putative genes encoding PHYTOCHROME A, PHYTOCHROME B, CRYPTOCHROME 2, COP1, LHY, CCA1, HY5 and GI (Fig. 6).
In total, 85 SNPs in genes associated with pigmentary, DNA repair, telomere/senescence and other pathways were successfully tested.
A total of 691 tagging SNPs capturing common variation in genes within the oestrogen synthesis and metabolism pathways were successfully genotyped.
PfPMT, and choline/ethanolamine-phosphotransferase (PfCEPT, MAL6P1.145; involved in the final step of synthesis of PC via the choline and SDPM pathways), were successfully detected [ 7].
Eventually, many communities, research groups and database developers got involved into the pathway reconstruction by collating experimental observations from published literatures and thus numerous different types of biochemical pathways were successfully mapped (1, 12).
In our cultured neurons, intracellular signaling pathways were successfully activated during shorter periods of exposition (<2 hours) in the presence of HEK293 cell supernatants containing soluble Cntns (unpublished data).
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Subsequently, 6 hexokinase genes, 3 glucose phosphate isomerise genes and 1 mannitol-1-P dehydrogenase gene involved in mannitol metabolic pathway were successfully cloned (Additional file 4: Figure S6), and corresponding proteins which expressed in E. coli BL21 were detected by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) (Additional file 4: Figure S7).
These results showed that the ED pathway was successfully constructed in S. cerevisiae, even though activity of the pathway was too weak to improve isobutanol biosynthesis.
The linalool synthetic pathway was successfully constructed by heterologously expressing a codon-optimized linalool synthase gene from Actinidia arguta in Y. lipolytica.
In the present study, the eight-gene FPP biosynthetic pathway was successfully expressed inside yeast mitochondria to enable high-level amorpha-4,11-diene production.
Alternatively, n-butanol synthesis pathway was successfully transferred into Escherichia coli and rapidly improved to reach a level of production comparable to the native producer.
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