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To better understand the interactions between chitin-mediated defense signaling and signaling mediated through SA, JA and ET the expression levels of key genes in each of the signal transduction pathways were monitored (Table S1).
Here, two signalling pathways were monitored by measuring phosphorylation levels of ERK1/2 and Akt, in IKVAV-treated BMMSC.
Accordingly, several enzymatic activities and metabolite pools located in these pathways were monitored, and a metabolic flux analysis was carried out at mid-stage meiosis.
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In the same study, the expression of genes, selected from cellular stress response (apoptosis, metabolism, proteolysis, chaperone, redox regulation, and DNA repair) pathways, was monitored.
To validate Affymetrix measurements, 32 genes related to "Electron Transport Chain" pathway were monitored using qRT-PCR, as explained in Methods.
In addition, polyamine levels and biosynthetic pathway were monitored in virulent Hessian fly larvae.
A series of genes in the central carbon metabolism pathway and TAG biosynthesis pathway were monitored, providing a comprehensive insight into how the cell is adapting to a specific condition as well as studying molecular changes that promote TAG hyperaccumulation.
Changes in regional cerebral blood flow (rCBF) and expressions of Angiotensin II and its receptors (AT1R, AT2R), as well as effector proteins in phosphatidyl inositol signal pathway were monitored before and at different times after MCAO.
This is not surprising, because the activation state of the PI3K signaling pathway is monitored by phosphorylated AKT.
Moreover, the photorespiratory pathway was monitored with respect to the 13C enrichment in the glycine, serine, and glycerate pools.
Transport of antitrypsin (A ) and VSVG (B ) along the secretory pathway was monitored by radioactive pulse chase assay.
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