The lumped biochemical reactions in the metabolic pathways are generated by stoichiometrically coupling several sequential elementary reactions.
Electrons for the reduction pathway are generated by oxidation of molecular hydrogen, catalyzed by electron-bifurcating hydrogenases [ 16].
Extracellular beta-amyloid peptides secreted from this pathway are generated by beta-secretase processing of amyloid precursor protein [ 34].
Other unique sperm isozymes in this pathway are generated by alternative splicing, including hexokinase 1 variants (HK1_V1 and HK1_V2) [ 5- 7], ALDOA_V2 [ 4], and a pyruvate kinase muscle form isozyme (PK-S) [ 8].
According to the KO assignment, metabolic pathways were generated with tools supplied by KEGG [ 31].
The representative pathways were generated using pathway analysis tool, by Fisher's Exact test and the C. reinhardtii KEGG database (http://www.genome.jp/).jp/
The data set was mined for significant pathways with the IPA library of canonical pathways and networks were generated by using IPA as graphical representations of the molecular relationships between genes and gene products.
All seven HIV-1 RT mutants from an AZT-resistance pathway (Figure 1) were generated by shuffling PCR.
Similarly, the top ten KEGG pathways enriched for miRNA target gene were generated by the same tool for each CNV subgroup.
