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In the endocytosis inhibition study, we found that the internalization of CS/DNA complexes took place via macropinocytosis and caveolae-mediated pathway; by incorporating γ-PGA in complexes, both uptake pathways were further enhanced but the caveolae-mediated pathway played a major role.
Pathways were further mapped to individual proteins (with assigned KOs) using MinPath [ 34] (Additional file 3: Table S3).
The associations between altered genes or pathways were further evaluated using the Ingenuity Pathways Analysis software (IngenuityR Systems).
For analytic purposes, the 286 pathways were further classified according to their functional groups as given by MetaCoreTM.
Internal regulatory pathways were further quantified by breaking up flux control coefficients into partial flux control coefficients.
Four genes (E2F2, SOX11, MAP3K4, and HSPH1), which represent critical nodes in these pathways, were further examined by real-time PCR validating differential expression between SCLC and NSCLC.
Similar(41)
The metabolite pathways are further divided into metabolic and non-metabolic pathways for simplicity.
Enriched pathways are further clustered into larger functional categories according to the GeneGO annotation.
The clathrin-independent pathways are further classified into caveolar and GPI-anchored early endocytic compartment (GEEC) pathways [ 32, 33].
The potential pathways are further verified using transcriptional units provided by the BioCyc Collection of Pathway and Genome Databases.
Besides lipids, it has been discovered that the dys-regulation of complement pathways is further responsible for AMD pathology [ 16].
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