Exact(42)
Upon identification of such pathways, we performed more in-depth analysis to characterize the identified pathways using coincidence imaging and time-resolved studies.
Finally, to experimentally confirm the interaction between miR-146a-5p miR-146a-5p miR-146a-5p, miR-146b-5pand genes of the MAPK/Wnt signalling miR-425-3p miR-425-3p miR-425-3pl experiments using miRNandimics on human embryonic kidney cells (HEK293).
For a global view of IFN signatures and regulatory pathways, we performed gene expression and chromatin analyses of the IFN-induced response across a range of immunocyte lineages.
To better characterize the possible interactions between Zn and Cd translocation pathways we performed two set of experiments.
To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait.
For each gene involved in these pathways, we performed a t test to compare the expression level between the healthy controls and the critically ill patients diagnosed with sepsis or systemic inflammatory response syndrome.
Similar(18)
To identify previously uncharacterized components of this pathway, we performed comparative, ex vivo, genome-wide RNA interference screening.
To understand how PLX4720 activates the autophagy lysosomal pathway, we performed quantitative PCR (qPCR) using mRNA extracted from PLX4720-treated A375 cells.
To examine the nature of the conduction pathway, we performed 110-ns molecular-dynamics simulations of two of these structures in a lipid bilayer with and without regulatory ions.
To further validate our experimental system of purification of cell populations at different stages in the apoptotic pathway, we performed measurements of caspase-3/7 activity.
To determine the 50%% inhibitory concentrations (IC50) of these primary hits on Wnt signaling pathway, we performed secondary screening using three concentrations by two-fold dilution, with the highest concentration being 20 μM.
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