We found no differential expression of target proteins, nor of the modulators of the apoptotic pathway tested in this study between the sensitive and resistant cell lines.
We found that the hormone pathways tested appear to be complex in their promotive and repressive roles Furthermore, there appears to be a cross-regulatory effect between GA and BR signals.
Functional activity of the TGFβ- and BMP pathways was tested in the chondrosarcoma cell lines SW1353 and JJ012 using luciferase reporter assays with two reporter plasmids carrying pSmad2 (CAGA-luc) and pSmad1 (BRE-luc) responsive promoter elements.
Our trained model was applied to all gene-pathway pairs tested in these RNAi screens.
The clinical pathway was tested in five selected hospitals before the implementation in all Lazio hospitals.
Proliferation, colony formation and activation of the WNT pathway were tested in cells after VANGL2 overexpression.
However, as some patients might receive romiplostim or eltrombopag after rituximab, this treatment pathway was tested in sensitivity analysis.
In year 2, students were taught with a realistic representation of the pathway and tested in the same way described above.
The effect of the laminin-332-blocking antibody on PI3K pathway was tested in vitro using TE10, which secreted laminin-332 at the highest level among cell lines and had high PI3K activity in vitro.
A total of five abiotic stress types, i.e. heat, cold, NaCl, PEG and H2O2, which all can be activators of MAPK cascade pathway were tested in this study.
