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It would be useful to engineer the lipid synthesis pathways existing in the chloroplast.
Our similar observations with recombinant TMVFN20 and TMVSC1754 [10] on Samsun nn leaves allowed us to study the mechanisms of some unknown resistance pathways existing in so-called susceptible tobacco hosts that could lead us to improve the technology of expressing more foreign peptides by the TMV-based vector.
Enzymatic information-based approach focuses on combination of gene knock outs and additions of pathways existing in different organisms [ 5, 6].
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Pyridoxal phosphate synthesis proceeds through the proteins encoded by pdxS and pdxT [ 24] and not by the pathway existing in E. coli and many other bacteria.
Since GIs suggest the existence of parallel pathways, we investigated the possibility of functionally redundant pathway pairs existing in the pathways we identified.
Although there are some natural biosynthetic pathways of trans-Hyp existing in microorganisms, the yield is still too low to be scaled up for industrial applications.
Since the stand-alone version of GO-Elite does not work for an over-representation analysis of KEGG pathways, we first downloaded the KEGG gpml archive from the PathVisio website (http://www.pathvisio.org), replaced other pathway flat-file relationships already existing in GO-Elite with that of KEGG, and then were able to perform a functional analysis of KEGG pathways in GO-Elite.
The biosynthetic pathways for fatty alcohols are diverse and widely existing in nature.
Both reconstitution of omega-3 LC-PUFA biosynthetic pathways and modification of existing pathways in microorganisms have demonstrated the potential to produce high levels of omega-3 LC-PUFAs.
This makes it easier to integrate biological pathways in existing applications and provides a framework for pathway-centered analysis of experimental data.
Understanding the logic of plant natural product biosynthesis is important for three reasons: it guides the search for new natural products and pathways, illuminates the function of existing pathways in the context of host biology, and builds an enabling 'parts list' for plant and microbial metabolic engineering.
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