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To identify specific metabolic and signaling pathways encoded in the transcriptome, we mapped mouse-annotated M. angustirostris genes to KEGG pathways [ 56].
To investigate the high levels of 4-methyl-5-thiazoleethanol in B. pseudomallei, we compared the thiamine degradation pathways encoded in genomes of B. pseudomallei and B. thailandensis.
Functional enrichment analysis of the collected genes was then performed using Gene Ontology (GO) and predefined canonical pathways encoded in MetaCore™ (GeneGo, Inc ., a commercial integrated knowledge database.
The 347 biological pathways encoded in E. coli K12 are retrieved from EcoCyc [23] and expression data for each gene under 466 growth conditions from the M3D database [24] are used to estimate the activation frequency of each pathway.
The Beacon Pathway Editor consists of a tool designed to draw pathways encoded in the Systems Biology Graphical Notation Activity Flow language that is a standard for describing pathways in terms of perturbations, influences, activities, logical operators, and phenotypes [ 41].
It was first assumed that variation in resistance resulted from immunological pathways encoded in the aphid genome, but later studies found that infection with heritable bacterial symbionts was responsible for a substantial portion of the observed variation [ 39].
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Although Oceanospirillales has repeatedly eluded cultivation efforts (Mason et al., 2012; Gutierrez et al., 2013), sequencing revealed the dominant pathway encoded in the core single-cell genome for this taxon was cyclohexane degradation.
These are described in Supplementary Tables 1 and 2. The pathway encoded in pLM495 was initially assembled using VEGAS (versatile genetic assembly system)19, and loxPsym sites were subsequently introduced between each pathway gene through PCR reactions using primers that introduced loxPsym sites and terminal, inward pointing BsaI sites.
In particular, the increase in relative abundance of a Colwellia, closely related to C. psychrerythraea strain 34H, a facultative aerobe, with the denitrification pathway encoded in its genome (Methé et al., 2005), suggested that facultative aerobes were present in the surface sediments.
In contrast to those studies, the mutation frequencies obtained from our MMR deletion mutants showed that the bacterial-like MMR pathway encoded in the genome of H. salinarum NRC-1 is not essential for maintaining the low genomic mutation rate we observed in this organism.
The available data strongly support the hypothesis that GBS haemolysin and GBS polyenic pigment share a common metabolic pathway encoded in the cyl operon and that the haemolysin is a molecule closely related or identical to the granadaene polyenic pigment.
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