In previous work, a chloroplastic GlyBet synthesis pathway was inserted into tobacco (which lacks GlyBet) by expressing spinach choline monooxygenase (CMO).
An expression cassette containing 13 genes including Clostridium phytofermentans XylA, encoding D-xylose isomerase (XI), and enzymes of the pentose phosphate pathway was inserted in two copies in the genome of Ethanol Red.
NPC assembly in postmitotic cells or in organisms such as yeast that undergo a closed mitosis, in which the nuclear membrane does not break down, occurs by a de novo pathway in which NPCs are inserted into an intact NE (Hetzer and Wente 2009).
Unlike artificial noses, heated humidifiers are inserted in the inspiratory pathway of the ventilation circuit.
Most proteins are inserted into the membrane by the well-conserved Sec pathway, consisting of a membrane-spanning translocase SecYEG in bacteria and Sec61 in eukaryotes.
With acupuncture, fine needles are inserted into specific points on the body that lie along meridians, or energy pathways, to re-balance the flow of energy and eliminate the blockages that are allegedly causing problems.
Fine needles are inserted into different points, either to stimulate or reduce the flow of energy along pathways.
While these pathways can be inserted into the model, we note that in the case of the NtA construct, synergy must occur completely through nascent branch targeting, suggesting nascent branch targeting may be the dominant displacement pathway for the full-length protein.
This study applies the constructal design method to obtain the configuration that provides the easier access to the heat flowing through an I-shaped high conductive pathway which is inserted into a volumetric medium with low thermal conductivity and heat-generating.
Recent work [ 29] followed a full synthetic biology design cycle, including computational analysis and full genome-wide flux analysis, to develop a metabolic pathway that was inserted into E. coli.
