4 5 Previous studies have identified variable genes, members of CHD-associated signalling pathways and single-nucleotide polymorphisms (SNPs) that are associated with an increased risk for CHDs.
Herein, "multi-pathway proteins" were defined as those involved in several cellular pathways, and single-pathway proteins as those involved exclusively in one.
In this study, we propose a new method that distinguishes gene markers with a small number of samples from case-control studies (especially, in knockout studies), by combining multiple methods such as DEG, gene regulatory network (GRN), biological pathways and single-nucleotide variants (SNVs), in a single computational framework.
Dihal, A. A. et al. Pathway and single gene analyses of inhibited Caco-2 differentiation by ascorbate-stabilized quercetin suggest enhancement of cellular processes associated with development of colon cancer.
One single miRNA can regulate hundreds of mRNAs in interrelated gene pathways, and a single mRNA can be targeted by several different miRNAs (Lewis et al. 2005).
One single miRNA can regulate hundreds of mRNAs in interrelated gene pathways and a single mRNA can be targeted by several different miRNAs [ 50].
Interestingly, while the degradation cluster only contains purine and pyrimidine degradation pathways and a single pyrimidine salvage pathway, the biosynthesis cluster also contains arginine biosynthesis pathways.
MiRNAs affect a variety of cellular pathways, and a single miRNA can target multiple genes [ 20, 21].
This structure reflects the principles of a bow-tie structure by having a hub node as center part, two short catabolic pathways, and a single anabolic pathway.
Signaling molecules can elicit different signaling pathways, and a single receptor can also respond to more than one signal molecule [ 8].
To date, those studies that addressed the process of human hematopoietic commitment focused on one or two differentiation pathways and on single or very few microRNAs without attempting to explore any extensive correlation between mRNA and microRNA expression profiles in multiple samples.
