Sentence examples for pathway were performed using from inspiring English sources
Functional annotation enrichment analyses for GO and the KEGG pathway were performed using the KOBAS server68.
Correlations between members of the pathway were performed using a Spearman's rank test.
The metabolic pathway was performed using Kyoto Encyclopaedia of genes and genomes.
Further analysis of the PIK3CA/mTOR pathway was performed using immunohistochemistry for the pAKT1, pS6 and p4EBP1 biomarkers.
The functional annotation of these human genes in the functional category and KEGG pathway was performed using DAVID Bioinformatics Resources [ 38].
Inhibition of the MAPK pathway was performed using p38 MAP Kinase Inhibitor III (Calbiochem, La Jolla, CA, USA) at 1 μM and JNK-inhibitor SP600125 (Sigma-Aldrich) at 5 μM.
In addition, besides studying the complete secondary metabolite network a focused study on terpenoids backbone biosynthesis (TBB) pathway using mevalonate and non-mevalonate pathway was performed using KEGG (map00900).
A statistical enrichment analysis of functional categories and pathways were performed using Ingenuity Pathway Analysis system IPA 6.0 (Ingenuity Systems, www.ingenuity.com).com
Given the involvement of INT6 in cell proliferation [16], western blots using a panel of antibodies against proteins involved in the cell cycle and associated signalling pathways were performed using lysates from control and INT6 siRNA transfected MDA-MB-231 cells.
Enrichment analysis of bio-pathways were performed using DAVID 6.7 bioinformatic resources [ 61].
Enzyme prediction and mapping to KEGG biochemical pathways were performed using BLAST2GO and PRIAM (Claudel-Renard et al. 2003).
