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As such, no comparison across the entire AFL participation pathway was conducted.
Inhibition of the classical pathway was conducted as previously described by Palarasah et al. [28].
Expression profiling of genes in the milk oligosaccharide metabolism pathway was conducted by next generation RNA sequencing (RNA-Seq) using Illumina IG technology [31].
Genotyping of tag SNPs from 48 candidate gene regions involved in TNF/NF-κB pathway was conducted at the NCI Core Genotyping Facility Advanced Technology Centerr, Gaithersburg, MD; http://snp500cancer.nci.nih.gov) [45] using a custom-designed GoldenGate assay (Illumina, www.illumina.com).
A final re-analysis of the pathway was conducted to ensure all objects were still connected to the main pathway.
Gene set enrichment analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) [ 8] pathway was conducted to find additional supporting evidence as described in Table 2.
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Interventions, centered on a clinical pathway, were conducted at Dartmouth-Hitchcock MedicaLebanon, New Hampshiren, New Hampshire).
Later experiments to find and investigate the hydratase described in this pathway were conducted by us, using Alicycliphilus denitrificans DSMZ 14773 as the bacterial source (Jin et al. [2010]; Jin et al. [2011]).
Unilateral transections of the right-side dorso-lateral funiculus (DLF) including the rubrospinal pathway were conducted at the C3/C4 intervertebral spinal cord level (Supplemental Fig. S1A and S1B).
Additionally, time course experiments for p-c-Met and active β-catenin, a transcription factor in the Wnt pathway, were conducted.
Enrichment analysis for GO terms and KEGG pathways was conducted by using LRpath.
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