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The miR-29a pathway was assayed by real-time PCR, western blot and chip analysis.
The promoter regions of genes related to the cucurbitacin pathway was assayed for the VOZ cis-element, GCGTNx7ACGC.
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Finally, it is worth mentioning that most assays that have been constructed to identify genes that affect recombination were applied without knowing the precise recombination pathway being assayed.
Components of the mTOR pathway were assayed by Western blotting in postmortem fusiform gyrus samples from 11 subjects with idiopathic autism and 13 controls and in valproic acid versus saline-exposed rat neocortex.
To determine whether the contribution by endophytic fungi to plant Taxol was the result of direct fungal biosynthesis of Taxol or elicitation of plant Taxol biosynthetic enzymes, expression of key genes in the plant Taxol pathway were assayed following the fungicide treatments in both young plantlets and mature wood.
To dissect the signaling associated with the cell-death and cell-cycle phenotypes observed in Figures 2 and 3, the impact of AGR2 knockdown on critical regulators of these pathways was assayed with Western blot.
To investigate the molecular mechanism underlying GM3S-mediated migration and invasion, some metastasis-associated signaling pathways were assayed by immunoblotting.
In addition, selected genes in the affected pathways were assayed by qPCR on a larger sample size to confirm the microarray data (see below).
Furthermore, the effect of α-ZAL on mitochondrial pathway-related proteins was assayed.
The effect of Mpi loss on phosphomannomutase 2 (Pmm2), the next downstream enzyme in the mannose metabolism pathway (Fig. 1A), was assayed.
The overall combined activity of the lower part of the pathway including XylD, XylX and XylA was assayed by following the formation of NADH using 80 mM d-xylonate as substrate (Meijnen et al. 2009).
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