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The RTK pathway intersection the authors observed in MEPM cells could easily be thoroughly validated in the background of the respective mutants.
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In the next section, we present the experimental results and analysis of the pathway intersections.
The main purpose of performing pathway intersections is to determine whether different cancers have identical chemoresistant mechanisms.
Although ovarian and lung cancers are assorted malignancies, based on the results of the pathway intersections experiment, several mechanisms are together responsible for platinum-based chemoresistance.
The advantages of pathway intersections analysis include: revealing whether different cancers have same chemoresistant mechanisms, and determining whether some common genes involved in these chemoresistant mechanisms.
This is defined as the ratio between the numbers of genes shared by any two pathways (intersection) divided by the number of unique genes in the two combined pathways (union).
The failure of the pathway enrichment-based method to match our network-based approach is somewhat expected, considering that many metabolic pathways do cross compartmental boundaries (as discussed above) and as it does not account for pathways intersection as shown below.
For a given pathway, the intersection of the set of genes up-regulated by experimental activation of the pathway with the set of genes showing anticipated patterns of expression in human tumor tissue specimens would be a set of genes of potential interest to researchers.
But even ants can get it wrong; sometimes a whole group of ants may bolt for a single exit, when more than one is available, and individual ants can panic, blocking pathways or intersections, making it hard for others to escape.
Additional research will be necessary to enable us to understand the complicated pathways and intersections linking community design, transportation, and a variety of health outcomes.
Table 4 lists some of the top significant pathways of these intersection genes.
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