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OP attachment to the serine residue represents the predominant if not definitive chemical pathway for analysis.
Therefore we first generated in silico signaling pathway, for analysis of important signaling pathways involved in early vertebrate development and performed analysis on the ERK1 and ERK2 transcriptome signatures using the GenMAPP software program.
We selected genes that encode the human IT signal transduction pathway for analysis by searching the literature for known human orthologs of those genes included in our prior analysis of the Drosophila IT pathway (Alvarez-Ponce et al. 2009).
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The MetPA is based on several databases and aids in identifying the top altered pathways for analysis and visualization.
From those pathways, we selected the fatty acid biosynthesis pathway for deep analysis.
The most significant pathway for this analysis was IGF-1 signaling pathway with p-value < 1.2 × 10-4.
For pathway analysis, Ingenuity Pathway Analysis software (version 8.5) was used (Ingenuity Systems, CA, USA).
Therefore, we have selected the lignin and cellulose metabolic pathways for further analysis.
The processing pathways for univariate analysis and MVPA diverged after these common steps (Fig. 2).
Corrected p-value cut-off of 0.05 was used to obtain functional categories and KEGG pathways for each analysis.
We thank Jennifer Meyers for her technical assistance in the RNA-seq, Dr. Wayne Yu for pathway analysis, Dr. Michael Goggins for providing PANC-1 cells, and Urvi Patel for technical support.
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