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Here the authors generate a library of 168 bidirectional promoters for the yeast K. phaffii (syn. P. pastoris) with diverse expression profiles to optimize metabolic pathway design.
We also put forth a systematic workflow that can be implemented in projects requiring pathway design and highlight current limitations and obstacles in computational pathway design.
Interviews were conducted to explore pathway design characteristics preferred and disliked by senior users.
We re-visited the pathway design and the priority grid that led to its adaptation.
Topics of interest include metabolic engineering, novel microbial pathway design and optimization, synthetic biology, and applications of molecular biology to bioprocess development.
H.Wi and M.C contributed to the conception of the manuscript, the drafting of 'Backcasting using long-term benchmarks' and 'A Paris-compatible pathway design framework' sections, and to revisions of the manuscript.
On-site observations were utilized to collect data on senior walking behavior (dependent variable) and pathway design characteristics (independent variable).
Especially, we discuss and highlight recent strategies for combining pathway design and protein engineering for the production of novel products.
We have developed a pathway design and optimization scheme that accommodates genetically and/or environmentally derived operational constraints.
These methods address many aspects of microbial fuel production: pathway design and manipulation, protein activity, and host engineering.
The outputs are also quantitatively ranked to show the preference for determining overexpression strategies in pathway design.
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