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METHODS: After exposure of sensitive and resistant sarcoma cell lines to imatinib for 24 to 48 hours, the changes in gene expression were evaluated using a 1146 unique pathway array with Western blot validation.
Samples of cDNA were then analysed using the Insulin Signalling Pathway Array (SABiosciences).
qPCR of WNT pathway members was performed using the RT Human WNT signaling pathway array (Qiagen).
The 20 pathways were assembled from the Ingenuity Pathway databases and the SuperArray cancer pathway array annotations.
The human mTOR RT profiler PCR pathway array (SABioscience Qiagen, Cat.no.330231 PAHS-098ZD) was used to validate mTOR regulation identified through RNA seq.
Thus, it is also noteworthy to observe that the absolute number of significant genes on each targeted pathway array increased between week 4 and week 10.
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Mouse cell stress focused pathway arrays in a 96-well plate format (PAM-003A), compatible with an ABI 7000 real-time PCR machine, were used to assay cell stress gene expression (3 comparisons for YFP-16 and 3 for wild-type controls, each comparison composed of pooled whole spinal cord tissue from 3 individual 2 month old mice).
The present study has utilised PCR hypoxia-signalling pathway arrays to explore the integrated response of human adipocytes to low O2 tension.
In total, 11 transcripts (P < 0.05 and fold change > 1.5) of the 84 genes analyzed on the p53 pathway arrays were upregulated, and two were downregulated.
To test the effect of PITX2 knockdown on the Wnt pathway signaling program, we analyzed the gene expression pattern of genes in the Wnt pathway as well as other pathways, by qRT PCR using modified Wnt pathway arrays.
GE Array Q Series pathway arrays containing 112 sites each (3 blanks, 3 negative reference spots, 10 endogenous genes, and 96 pathway-specific human genes) were obtained from SuperArray Bioscience Corporation (Frederick, USA).
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