Detailed understanding of brain activation requires parallel assays of all substrates consumed by brain, fluxes in glycolytic, pentose shunt and oxidative pathways and quantification of products released from brain.
This approach allows for the identification of certain regulatory features of the pathway and for the quantification of the behaviour of the reaction blocks towards to changes in the concentration of the fixed metabolite.
Given the postnatally lethal phenotype of the Wnt5a−/− mouse, the midbrain dopamine pathways were examined developmentally and quantification performed at E18, an age when the DA pathway is established, axons have reached the striatum and numerous synaptic contacts made.
Topics will address unmet needs for visualization and quantification of molecular pathways in biology as well as for diagnosis and disease management.
In fact, signals generated from specific metabolic reactions and pathways are the basis for detection and quantification of changes in local functional activity because energy demand increases the flux of glucose into and through these pathways.
Molecular imaging can be defined as noninvasive visualization and quantification of distinct molecular pathways.
The CLUE-S model [28] explores quantitative, spatially explicit, multi-scale pathways of land-use changes through the determination and quantification of the most important driving forces.
Molecules from various cellular pathways, "intermediates", can serve as candidates for measurement and quantification [ 1, 35, 36].
The CLUE-S model [ 28] explores quantitative, spatially explicit, multi-scale pathways of land-use changes through the determination and quantification of the most important driving forces.
Characterization, dynamic detection and quantification of chemical modifications of the pathway components are essential for us to gain deeper insight into biological control of the pathway.
BACKGROUND: Metabolomics, the global science of biochemistry, is an emerging field that enables detection and quantification of small molecules involved in metabolic and signaling pathways.
