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This could be indicated by a higher total number of pathology observations per animal.
Median number of pathology observations per animal was increased in dual heterozygous animals compared with heterozygous loss of Trp53 alone.
Assuming pathology is included in the experimental plan from the start and the technical and cost hurdles surmounted, the ultimate challenge is to create meaningful data from the gross pathology observations and histopathology.
Case in point, the work of Baskin et al. [ 31] to collectively analyze microarray, clinical data and pathology observations revealed that gene expression patterns were very much consistent with the clinical outcomes, gross pathology and histopathology from influenza virus-infected pigtailed macaques primates.
As shown in Figure 5, pathology observations were recorded in multiple organs for most animals in all cohorts, including some of the wild-type littermates (Men1+/+: Trp53+/+), which generally reached very advanced age but nevertheless had often developed several minor pathologies.
The observation of highest median number of pathology observations per animal in the Men1+/−: Trp53+/− cohort indicates that tumours at more sites, likely corresponding to increased total tumour burden, was the cause underlying the steeper slope of the survival curve for these animals in comparison with either mutation alone.
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The other pregnant females were subjected to caesarian section between G17 and G19 for fetal survival and placenta pathology observation.
Data are shown as "Number of animals with abnormal or pathology observation"/"Total number of animals" (percentage of cohort), tabulated by genotype group.
First, the left panel shows the visible image of the tissue section part which was acquired by the FPA with the red line defining the borderline between the two areas identified by the pathology observation.
The xenograft growth curves of the 3 groups were based on the mean volume of 6 samples weekly, and inhibition was calculated based on the volume 5 weeks (the tumours of the 2 control groups at week 6 were too large for ultrasonography) after treatment as follows: At the culmination of therapy, tumour tissues were harvested and preserved in 10% neutral buffered formalin for pathology observation.
In concert with the discussed evidence of a sequential staging of PD pathology, these observations lead to the proposal that the progression of PD may be mediated by a prion-like process [ 25, 26].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com