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The patient demographics (age, underlying pathology, number of treated levels and duration follow-up) are summarised in Table 1.
(B) Severity of chondrocyte pathology: Number and distribution of chondrocytes in cartilage.
Due to the small number of patients in pathology number 5 (n = 79) and pathology number 6 (n = 61), these patients and the patients who could not be assigned to any pathology (n = 144) were merged into a separate category, pathology number 9 (n = 285), as shown in Fig. 1.
A maximum of 0.5 ml capillary blood was taken from each child into an EDTA tube labelled with the patient's barcode and pathology number.
A single pathologist (SF) with special interest in renal cancer, blinded to clinical outcomes, reviewed all the samples using pathology number identifier for each patient.
The test was performed following manufacturer's instructions [ 7]. 5 μl of well mixed capillary blood was placed on the dye labeled area of a slide with the patients' pathology number, cover slipped, incubated at room temperature for a minute and observed under the 100× objective (oil immersion) under LED UV light (365 nm).
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Among three founder lines identified with abnormal lung pathology (numbers 12, 29, and 51), one line (51) was particularly studied in further detail.
Hospital and pathology numbers.
Sequential bank identifiers are assigned by the ACSR database software to replace surgical pathology numbers and hospital identifiers in all communications outside the ACSR.
Patients not included in the present study were a result of missing blocks, lack of patient data, wrong pathology numbers or lack of residual tumour tissue in the collected blocks.
The percentage of each parameter was calculated by the following equation: Percentage of each pathology = Number of glomeruli involved × 100%% Total number of glomeruli obtained All biopsies were examined by one pathologist (WK) who was not aware of the molecular results.
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