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In this review, we discuss the continually evolving pathological characterization and in vitro mechanobiological characterization of tumor invasion, with emphasis on emerging physical biology and mechanobiology strategies that have contributed to a more robust mechanistic understanding of tumor cell invasion.
Pathological characterization of p23-ST1 mice showed marked astroglial and microglial reaction as well as a significant loss of neurons in septal areas (Fig. 8).
In the present study, we investigated the comparative proteome of prostate cancer and its adjacent histological benign tissue from cancer patients with definitive pathological characterization in our 2-D differential in-gel electrophoresis (DIGE) system for protein 2-D electrophoresis [13] and MALDI-TOF-MS/MS for protein identification.
A subset of cell lines (focused on the HCC series) was subjected to a more detailed molecular pathological characterization of ESR1, PGR, ERBB2, EGFR and BRCA1, as summarized in Table 2. Cells were grown to 70 80% confluence, then harvested for total RNA and genomic DNA.
HW participated in tumor pathological characterization.
QC and FW participated in tumor pathological characterization.
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CAHK performed all pathological characterizations.
JAH performed all pathological characterizations, BD performed FISH analysis on all specimens, HP carried out the genotyping experiments, studies, BL oversaw all statistical analysis, DLE and CDS contributed to the preparation of the manuscript and REE conceived of the study, and participated in its design and coordination and helped to draft the manuscript.
The clinico-pathological characterization of this GIST cohort is summarized in Table 3.
Clinico-pathological characterization of GIST used for Cleveland Clinic GIST TMA is summarized in Table 2. SuperBiochips GIST TMA was purchased from Super BioChips laboratories, (#ADD1, Seoul, South Korea).
Pathological and glycosphingolipid characterization revealed no discernible differences to the parental Fabry mouse line (data not shown), though differences in the rate of loss of heat-sensitivity (measured in the hot-plate assay – see below) were observed.
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