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The pathogens were grown to 0.4 of OD600nm at 37 °C in MHB medium and diluted to approximate 1 × 105 CFU/ml with fresh MHB medium.
The pathogens were grown in nutrient broth supplemented with 50 µg/ml prodigiosin in 96 well microtiter black plates and incubated at 30 °C for 24 h.
The pathogens were grown in nutrient broth (peptone 5 g/L; beef extract 3 g/L; NaCl 5 g/L; pH7.0) supplemented with increasing concentrations of prodigiosin (1 50 µg/mL) in 96 well micro titre plates and incubated at 30 °C for 24 h.
All pathogens were grown at 37°C except C. neoformans, which was grown at 30°C.
After the pathogens were grown to an early exponential phase with an optical density (OD) between 0.2 and 0.6, they were diluted to OD 0.02 with Mueller Hinton broth with the appropriate antimicrobial cocktails, which were freshly prepared before each experiment.
Therefore, 15 clinically relevant human pathogens were grown on Columbia sheep blood agar, and headspace of these cultures were analyzed by MCC-IMS.
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Both EB and pathogen were grown on King's B (KB) medium at 24 °C for 24 48 h (Schaad et al. 2001).
Pathogens are growing threats to wildlife.
In an increasingly connected world, the threat of spreading existing and emerging pathogens is growing (Daszak et al. 2000) and in some cases devastating (e.g. Jensen et al. 2002; Jancovich et al. 2004; Fenton 2012).
For 12 (8.5%) PVL + ve infections another pathogen was grown from the same specimen compared to 61 (41.2%) of the control group.
The pathogen was grown in NYGA liquid medium containing rifampicin (50 μg/ml) and kanamycin (25 μg/ml) as described above.
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CEO of Professional Science Editing for Scientists @ prosciediting.com