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In response to the demand for rapid on line or at site detection of pathogens, in this study, we developed a multiplex Direct PCR method for rapid detection of different Salmonella serotypes directly from pork meat samples without any DNA purification steps.
Nevertheless, further studies are needed in order to determine the specific substrate of the zinc-metalloprotease identified in WS coral pathogens in this study.
We also tested for other suspicious pathogens in this study.
Nevertheless, it was among the most common tested pathogens in this study.
Therefore, mixed infection with multiple pathogens in this study has been excluded.
RSV, MP, EV and HCoV-OC43 were the main co-detected pathogens in this study.
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lachrymans, the causative agent of angular leaf spot (ALS) on cucumber, was obtained from the "Collection Française des Bactéries Phytopathogènes" (CFBP, Angers, France) and used as the challenging pathogen in this study.
Species belonging to Enterobacteriaceae family were the second most frequent co-pathogens in this study, including even multidrug resistant, VIM-4 carbapenemase producing Enterobacter cloacae strains [ 25].
Therefore, we chose M. catarrhalis as the index pathogen in this study.
UTI89, a well-characterized and fully sequenced uropathogenic E. coli strain, was used as the prototypic pathogen in this study.
S. epidermidis was the most common causative pathogen in this study, which agrees with findings from previous reports [ 9, 10, 12, 13, 17, 20].
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