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Nonetheless, no general conclusion can be drawn regarding the impact of a carbapenem-based de-escalation strategy on the emergence of resistant pathogens based on this study, since the proportion of NP episodes empirically treated with imipenem varied significantly between institutions.
For these reasons, in a redefinition of the concepts of virulence and pathogenicity of microbes, Casadevall and Pirofski suggested a classification system for pathogens based on their ability to cause damage as a function of the host's immune response [ 58].
Calvin Chue, a research scientist at the Center for Civilian Biodefense Studies at Johns Hopkins University, said that in three to five years, so- called DNA chips, which contain snippets of genetic code, might be able to detect virtually all pathogens based on their genetic signatures.
Identification of pathogens based on their nucleic acid detection is very important in current clinical diagnosis.
A sensitive and selective genomagnetic assay for the electrochemical detection of food pathogens based on in situ DNA amplification with magnetic primers has been designed.
The Bruker BioTyper, a simple matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer for identification of bacterial pathogens based on intact protein masses, "has been a smash success ….
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However, over a dozen long oligonucleotide probes were designed for each pathogen in the EOPM method, allowing reliable identification of a pathogen based on a statistical enrichment analysis of the probe group, instead of one individual probe.
Our goal was to provide a complete, experimentally validated assay in a ready-to-use format, which could be applied generically to any pathogen based on genome target recognition.
The present strategy differs from previous comparative genomic screens for infectious diseases and for which the goal was to identify potential drug targets unique to the pathogen based on a user-prescribed similarity cut-off value in order to decrease the potential for toxicity of any new chemotherapeutic [35], [36].
A Wilcoxon rank sum test supported a separation of pathogens and non-pathogens based on the average size of singleton gene clusters (p=0.046) and the significance increased when further considering free-living non-pathogens only (p=0.003, Figure 9).
A more practical approach of classification is to consider the pathogen based on its clinical pattern of infection [ 2].
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