However, the evaluation of pathogenicity of this variant must be done with caution.
A susceptible cultivar of pepper (Capsicum annuum inbred line 06221), and tobacco (Nicotiana benthamiana) were selected from different inbred lines based on evaluation of pathogenicity after inoculation with zoospores of highly virulent P. capsici SD33.
The culture supernatant was filtered with a 0.22-µm filter and was then used to inoculate suckling mice intracebrally for an evaluation of the pathogenicity of the isolated virus.
The finding of variants in controls, particularly the finding of a nonsense mutation in a 69 years old healthy individual, clearly adds to the requirement of an extended evaluation of the pathogenicity of each variant.
Evaluation of the pathogenicity of IBV was based on several criteria, including: clinical signs, gross pathologic lesions, histopathologic changes, and tissue tropism.
The analysis, using a random-walk with restart (RWR) method, is adapted to the setting of WES by developing a composite variant-gene relevance score based on the rarity, location and predicted pathogenicity of variants and the RWR evaluation of genes harboring the variants.
Type I IFNs function as a double-edged sword that both control pathogens and exacerbate pathogenicity.
Polymerase chain reaction was used to evaluate the pathogenicity of these samples in the plants.
This mutation may be closely related to the pathogenicity of the pathogenic strains because the substitution occurs within the LexA binding sites [ 26]; these results indicate that it is worth performing an experimental evaluation in the future.
4 The Norwegian study presented in vitro evidence to support pathogenicity of the variants (but no familial evaluation).
