Exact(29)
Practices vary--for example, the Wellcome Trust requires grantees to release pathogen sequences as they accumulate, but data from the bacterium Bacillus subtilus, financed mainly through the European Commission, were not put out promptly.
In previous studies, the estimation of "contaminating" pathogen sequences was based on GC content [22], [36].
Probe and primer design was achieved by aligning URI pathogen sequences obtained from GenBank and finding suitable regions for amplification and detection of each organism.
We describe in detail an in silico approach to mine ESTs from inoculated host plants deposited in a database in order to identify particular pathogen sequences associated with disease.
We have implemented a bioinformatics strategy (Fig. 1) for the detection of putative pathogen sequences from large data sets of short sequence reads, such as those generated by the Illumina GAIIx and HiSeq.
The resulting sequences were analyzed with the blastn algorithm against the potato (Solanum tuberosum) and tomato (Solanum lycopersicum) EST sequences and against the Solanaceae ESTs deposited in the GenBank dbEST to test whether the identity ratio cut-off was being useful to separate host from pathogen sequences (Table 2).
Similar(31)
Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Cambridge, CB10 1SA, UK.
We thank the Wellcome Trust Sanger Institute core and pathogen sequencing and informatics teams who were involved in the Malawi and Uganda studies.
CCDD researchers have been at the forefront of efforts to use pathogen sequence data to infer who infected whom, and separately to enhance vaccine trial designs (see above).
To evaluate read mappings, BWA aln (BWA version 0.7.10-r789, http://bio-bwa.sourceforge.net/ 54 was used to align reads, and the "XA tag" was used for read enumeration and separation of host and pathogen sequenced reads.
And the plant pathogen sequenced, Xylella fastidiosa, is an insect-borne bacterium that has been ravaging Brazil's orange groves, causing their trees to produce shrivelled fruit and costing growers an estimated $100m a year.As if sequencing X.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com