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While there is no PO-BW polymorphism in the amplified region, both embryonic and placental bw×po clones displayed the expected 50∶50 methylated (maternal): unmethylated (paternal) ratio (Fig. 2).
(25% vs 10%, p = 0.04) The inheritance patterns were also examined by genotype, AT versus non-AT.The maternal ratio, paternal ratio and maternal inheritance ratios were similar in both AT and non-AT subgroups (Table 3).
The number of functional, neurological and endocrine conditions in both first- and second-degree relatives was determined and the maternal ratio, paternal ratio, and maternal inheritance ratio were calculated using established criteria.
RT-PCR and sequencing of RNPC3 transcripts from patient cell RNA showed the mutated alleles to be expressed in a 2 1 (maternal:paternal) ratio, likely due to partial nonsense-mediated decay (NMD) of paternal mRNA (Fig 2B).
But in reciprocal crosses between Col or Ler females and Cvi males, both maternal and paternal alleles are hypomethylated and the gene is biallelically expressed in the expected 2 1 maternal:paternal ratio.
We observed a gradual increase of paternal siRNA expression in the early stages of kernels and an expected 2 1 maternal to paternal ratio in 7-DAP endosperm via sequencing of small interfering RNA (siRNA) transcriptomes in developing kernels (0, 3 and 5 days after pollination (DAP)) and endosperms (7, 10 and 15 DAP) from the maize B73 and Mo17 reciprocal crosses.
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We measured 'circularity', which is 4 π × [ Area]/[Perimeter] and calculated the paternal:maternal ratio.
Figure 1 figure supplement 3 examines maternal:paternal ratios of imprinted genes identified in one set of crosses in the other two sets of reciprocal crosses.
A patient had a probable maternal inheritance (PMI) pattern if the maternal ratio was ≥1.75 and the maternal inheritance ratio (MIR) is ≥ 3 where the MIR is calculated by dividing the maternal and paternal ratios.
The data were then exported into Excel and the individual maternal to paternal ratios (male/female ratios) for each zygote calculated as this measure is widely used in the field and allows for a good degree of comparison between studies.
It is therefore possible to calculate a conservative (using the lowest estimate based on whole-genome bisulphite-sequencing, [ 38]) theoretical paternal to maternal ratio of DNA methylation of 2 for PN0.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com