Sentence examples for patching solution from inspiring English sources

In fact, Rusovsky says the development of Rox was influenced by feedback from its existing customer base, who had manipulated the hot patching solution to deploy new features safer and faster.

Furthermore, the internal patch solution was filled with secondary antibody alexa 488 to allow MN identification after an additional immunostaining step using alexa 555 against SMI32 primary antibody.

In perforated-patch experiments reported in Figure 3A, pipettes were tip-filled with patch solution containing ≤0.5 mg/mL amphotericin B (1% DMSO; Sigma).

Micropipettes were backfilled with internal patch solution containing (in mM) K-gluconate 110, KCl 10, HEPES 10, Phosphocreatine 10, Mg-ATP 4, Na-GTP 0.3, Biocytin 0.1%, pH 7.3, with and osmolarity of 280 290 mOsm.

Current-clamped recordings were obtained with the above internal patch solution consisting of 135 mM K-gluconate.

Some cones were recorded with an EGTA zero Ca 2+ perforated patch solution in the pipette (blue lines; see 'Results').

Biocytin (0.5 mg) was mixed into 1 ml of patch solution just before use and filtered as used.

For patch-clamp experiments, the drugs were dissolved at the desired final concentration in external patch solution containing up to 1% dimethylsulfoxide.

For pharmacological experiments, acetazolamide (ACTZ, Sigma-Aldrich) was first dissolved in dimethylsulfoxide (DMSO) then in the external patch solution at the desired final concentration.

In four of five cones recorded with 1 mM EGTA in the pipette (EGTA zero Ca 2+ perforated patch solution), we observed the same progressive increase in coupling.

We added 2 4 µl/ml of Alexa Fluor 568 hydrazide (Na+ salt, Invitrogen, Carlsbad, CA) to the patch solution for fluorescent visualization of morphology.