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For replication fork speeds, the lengths of red- (CIdU-AlexaFluor 555) and green- (IdU-AlexaFluor 488) labelled patches were measured using the ImageJ software (http://rsb.info.nih.gov/ij/).
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Currents through L-type Ca2+ channels were measured using the patch-clamp technique in the whole-cell configuration.
Exocytosis and ion channel properties were measured using the patch-clamp technique in INS-1 832/13 cells and beta-cells from miR-375KO mice.
Fifteen hours after challenge the mucosal production of nitric oxide (NO) and the release of myeloperoxidase (MPO) as signs of mucosal inflammatory reaction were measured using the mucosal patch technique.
In order to investigate the role of PKC in CCL2-induced inflammatory nociception, the effects of PKC on the peak current density of Nav1.8 were measured using whole-cell patch-clamp protocols.
Ionic currents through voltage-gated K+ channels were measured using whole-cell patch clamp electrophysiology, in the presence of the voltage-gated Na+ channel inhibitor, tetrodotoxin (TTX; 0.3 μM).
Open probabilities (Po) of the BK channel were measured using the cell-attached patch clamp technique.
Voltage dependent currents were measured using the whole cell patch clamp technique [ 37].
Whole cell currents were measured using an Axopatch 200A patch clamp amplifier and recorded using pClamp 9 software (Molecular Devices, Sunnyvale, CA).
Currents were measured using the whole-cell patch clamp technique and an Axopatch 200B amplifier in combination with Clampex 9.2 software (Molecular Devices, Sunnyvale, CA).
Currents were measured using the whole-cell variant of the patch clamp method at a temperature of 37 ± 1°C.
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