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The noisy character of deoxygenation-activated currents in our on-cell patch records resembles previously published patch records of ligand-induced nonspecific cation conductance in AA red cells [52], [47], and could be intrinsic to Psickle.
The on-cell patch records of Figure 1A, with NaCl in both pipette and bath, show that deoxygenation activates noisy channel activity in the SAD sickle mouse red cell membrane.
These data and those of Figure 5 are the first to document deoxygenation-activated conductance in on-cell patch records of individual human SS cells transitioning from room air to hypoxic conditions.
The whole cell conductances estimated from nystatin-permeabilized patch records from oxygenated and deoxygenated human SS cells of 135 µm2 nominal surface area were 1.0 x 10−3 µS cm−2 and 2.4×10−3 µS cm−2, respectively.
Every 2 min, the patch records a 30 s segment of ECG, followed by a 60 s segment of respiratory signal; thus the individual vital signs measurements are sequential, independent and non-continuous.
Similar(54)
The functional consequences of single proton transfers occurring in the pore of a cyclic nucleotide-gated channel were observed with patch recording techniques.
In vivo two-photon-targeted patch recording revealed that PVNs have exceptionally broad olfactory receptive fields and exhibit largely excitatory and persistent odor responses.
Furthermore, this approach allows subsequent targeted whole-cell patch recording based on well-defined connectivity as well as assessment of physiological activity in targeted circuits on a fast time scale.
We next demonstrated that the condition of whole-cell patch recording was stable and reliable when the PDMS was indented.
Whole-cell patch recording studies showed that spiral ganglion neurons respond to protons and generate inward currents.
Positive experimental evidence for the pattern III or VI distribution of potassium conductances could be approached either electrophysiologically by focal patch recording or anatomically by immunocytochemical procedures.
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