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The Patch experiment, a huge collection of local sites around the country, is faring poorly, and its owner, AOL, has been less and less supportive.
Manipulating color to be less salient while enhancing the saliency of the orientation of the Gabor patch (Experiment B) led to delayed color selection and enhanced orientation selection.
Unfortunately, isolation of interstitial cells for patch experiment in HGCS is technically limited.
DOI: http://dx.doi.org/10.7554/eLife.03222.012 To explore the relationship of the GF patch (Experiment 1) to the face patches found in Experiment 2, we projected the BOLD patterns obtained in the two experiments onto coronal sections and onto a partially unfolded 3D representation of the individual monkey's brain, based on anatomical MRI images.
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Voltage protocols were similar to those described for cell-attached patch experiments.
Holding potentials in on-cell patch experiments were expressed as −Vp, the negative of the pipette potential.
This application solution was used in all the nucleated patch experiments and was applied directly to the patch using a glass pipette.
In perforated patch experiments, we waited 15 45 mins after establishing a giga-ohm seal before beginning an experiment to allow the series resistance to fall below 50 MΩ.
The ionic conditions of our on-cell patch experiments could not rule out a contribution of Cl− conductance to the observed deoxygenation-activated currents, but suggested (Figure 1C) such a contribution is minor.
Whole-cell voltage-clamp and cell-attached patch experiments were performed using an Axopatch 200B amplifier (Molecular Devices, Sunnyvale, CA, USA), Digidata 1322A acquisition board (Molecular Devices), and pClamp software (Molecular Devices).
The capsaicin current desensitized but remained large during the application period, suggesting that rapid desensitization of capsaicin-induced current is not likely to be responsible for the abbreviated spiking observed in cell-attached patch experiments.
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