Similarly, sco1-1 seedlings that were able to survive when germinated in soil had large green patches (data not shown).
In lymphoid tissues, ME-9F1 marks the endothelium of arterioles and capillaries in the spleen and the high endothelial venules in lymph nodes (Fig. 2a, lower panel) and within Peyer´s patches (data not shown).
Reversibility measured by the QPatch instrument did not match reversibility values obtained by classic patch clamp (data not shown).
However, at homeostasis, IL-10 mRNA levels were similar in the MLN (Fig. 4C), such as for jejunum and Peyer's patch samples (data not shown).
Similar to the results reported by Vocke et al. (2013), the current amplitudes of these mutant channels were much smaller compared to wildtype channels in our inside-out patch recordings (data not shown).
The sudden baseline shifts did not occur in cells treated with NPPB prior to patch-clamping (data not shown).
Similar to CHO hERG cells, growing HEK293 hERG cells at 30°C also increased hERG current density measured by patch clamping (IonWorks, data not shown), and specific H-dofetilide binding (Fig. 4C).
Indeed, a 10-min continuous application of 25 μM GABA to P4 control neocortical slices generated a persistent current in patch-clamped neurons (data not shown).
In female ILA and ACAd, no significant change in the number of cells expressing CREB and pCREB was found (Fig. 3 B, D ) after chronic and acute stress, also no patches were present (data not shown).
DOI: http://dx.doi.org/10.7554/eLife.02949.006 All R1 experimental and control colonies (20 of each) had lost pSC101TS- NEW1 or pSC101TS, respectively, as assessed by patching on selective medium (data not shown).
