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Cells past passage 3 were grown in 10 % female bovine serum in culture media.
3T3L1 mouse embryo fibroblasts (ATCC) were cultured according to the supplier's instructions and were not used past passage 10.
Compared to primary cultures that senesced at passage 10 and were no longer viable by passage 12, the immortalized cells were growing well past passage 25.
Twenty out of 27 pleural effusion samples (74%) produced viable mammospheres (20 to 100 μm) that could be cultured past passage 2 (Table 1).
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As for the Stasi past, the passage from amnesia to awakening is still in progress, promising further traumas down the road.
About the Reporting Sandra Ochoa, a newspaper reporter for El Tiempo of Cuenca, Ecuador, who has contributed to The New York Times in the past, arranged passage on the fishing boat William at the request of The Times after its correspondent Ginger Thompson was told by migrant smugglers that they would not take her.
We have now conducted the RT-PCR analyses on the avian cells that have made it past the 5th passage, sampled at the 12th passage, and demonstrate that these cells maintain expression of the endogenous genes several months after being induced and at least 7 passages post-exogenous gene silencing.
This property may reflect the past history of passage of this strain that was artificially passaged in eggs and then subsequently in the brains of mice.
"It is the product of work informed by a sense of urgency given the tension and violence of the past year and the need to contend with the past before the passage of time makes this even more difficult," they said.
Do the same north-south clichés hold there, but inverted by passage past the equator?
So now watch out for the Chinese reaction when US warships make innocent passage past Fiery Cross or Mischief Reef.
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