DLD is a popular passive sorting technique for microfluidic applications.
Active sorting using acoustic forces can be improved by combining it with passive sorting methods.
Subsequent purification of such tissue digests based on size and deformability, whether by active or passive sorting techniques, may remove debris and unwanted, terminally differentiated cells while yielding these enriched subsets.
Future developments could include the combination with on-chip functionality or other optical techniques, such as Raman [ 41] or fluorescence spectroscopy [ 40], or optical landscapes for active or passive sorting [ 43], before and/or after photoporation.
By L. N. Freschl and Russell Maloney The New Yorker, October 29, 1938 P. 13 At the threat of war, the command went out for the mobilzation of the defense passive, a sort of home guard detailed to keep order in Paris during bombings.
He's always been the passive thinker sort (not a ton of energy, not super athletic, not super social, great with pattern recognition/ problem solving/ reading/ creativity).
The combination of microfluidic photoporation with on-chip sample mixing, cell culture or analysis or alternatively microfluidic sorting schemes, such as fluorescence/Raman activated cell sorting (FACS/RACS [ 40, 41]) or passive optical sorting [ 42, 43] can now all be envisaged.
For most of my travel career, I had spurned such tours; they seemed like cop-outs, perfect for passive, obedient sorts who wanted their adventures prescreened.
Brent Skidmore, a fellow student, added that one lesson from the melodrama was clear: "It shows the system needs more support than the normal passive-aggressive sort of voter we have today".
