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The calculated passing velocity of IgG molecules based on a simplified model will first increase, then decrease, and finally stabilize with the increasing IgG concentration, which can provide support for our experimental results.
Solid black points stand for the number of IgG molecule passing the nanopores in one simulation step (10,000 step approximately 10 ps) and the blue line in the points is the average curve which corresponds to the average passing velocity of IgG.
It can be found that with the increasing IgG concentration, the calculated passing velocity (the passing number in one simulative step) of biomolecules will not increase continuously but will increase at first, then will decrease and will finally stabilize.
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Figure 7 The calculated passing velocities of IgG molecules changing with IgG concentration.
In this way, other velocities at different IgG concentrations can be obtained (the detailed results can be found in Additional file 1), and the calculated passing velocities of IgG molecules changing with IgG concentration can be plotted as showed in Figure 7.
To determine how velocity varied over shorter time scales we employed a rolling temporal window of 15 frames (33 ms) duration, which is equivalent to passing the velocity signal through a lowpass filter.
Open image in new window Figure 8 Velocity vectors just passing the upper frame.
Gas velocity passing through the cluster surface varied significantly at volatile liberation.
In PRDOCT, the phase difference between A-lines acquired at the same spatial location is related to the blood flow velocity passing through probing beam.
The technology needs to handle the transition from the vacuum of space at hypersonic conditions, decelerating to supersonic velocities and passing through transonic buffet, before relighting one of the main-stage engines at terminal velocity.
Three starts have passed without his velocity increasing or his command improving, and it is natural to wonder how the Yankees will react.
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