Sentence examples for passing cell from inspiring English sources
We observed several steps in traditional RNA extraction workflows to have a detrimental effect on RNA yield from minimal cell numbers, for example passing cell lysates through homogenisation matrices or carrying out on-column DNAse I treatments.
After passing cell homogenates 10 times by a insulin syringe, extracts were left on ice for 15 min and centrifuged at 14.000 xg for 5 min at 4°C.
Cell pellets were then resuspended (0.5 ml/10 cm dish) in homogenization buffer [10 mM MOPS, pH 7.0, 10 mM potassium chloride, 1× complete protease inhibitor (PI)] (Roche Molecular Biochemicals, Germany), and homogenized by passing cell suspensions through a 23-gauge needle 10 times.
Infected and uninfected macrophages were lysed with TRIZOL® Reagent (Invitrogen) and homogenized by passing cell lysate through a pipette.
Proteins were purified by passing cell extracts through Ni-NTA (Ni2+-nitrilotriacetate) resin pre-equilibrated with buffer A and eluted with buffer B (buffer A and 250 mM imidazole).
There are many ways to hold her attention, including making eye contact, using gesturing and body language (e.g., facing the person, not having your arms or legs crossed), speaking clearly, and avoiding outside distractions (e.g., background noise, people passing, cell phones ringing).
One has a loop projecting outward that's easily discerned by passing cells.
After transfection, the cells were placed in media containing 800 ug/ml Geneticin and cultured for 14 days passing cells every three days with fresh media and Geneticin.
By measuring side-scatter signals emanating from passing cells, the degree of cytoplasmic granularity or size of lipid droplets of single cells can be obtained.
After transfection the cells were placed in media containing 800 ug/ml Geneticin and cultured for 14 days passing cells every 3 days with fresh media and Geneticin.
After passing cells 20 times by a insulin syringe, extracts were left on ice for 15 min and centrifuged at 20.000 xg for 10 min at 4°C.
