Most passenger strand modifications will not negatively affect RNAi activity as long as the A-form helix structure of the siRNA duplex is maintained.
Therefore, a method for various passenger cars without significant modifications is needed.
No production freighter version of the 747-300 was built, but Boeing began modifications of used passenger −300 models into freighters in 2000.
Interestingly, unlike the guide strand, modifications of the passenger strand, such as chemical modification and introduction of bulge structures, are well tolerated.
In addition, the passenger strand may contain chemical modifications to prevent RISC loading, while it is further left unmodified to ensure rapid degradation (Chen et al, 2008).
Karsan appeared to be staking much of its bid on the V1's ability to load and carry a passenger in a wheelchair without modification.
On the other hand, the modification of siRNAs with cholesterol at the 3′-end of the passenger strand was tolerated; however, the modification at the guide strand significantly reduces its silencing activity.
Mr. Webb bought USA 76 in 2010, made minor modifications to convert it to a passenger vessel and began public sailings in June 2011.
In particular, the 2′-azido group possesses the extraordinary property of being well accepted in the guide (antisense) strand, while most other modifications are much better tolerated in the passenger (sense) strand.
In the short term biofuels are looked on as a potential savior as most commercial passenger jets can use them with little to no modification, and because they seem to offer significant benefits.
