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Within our data sets >80% of analysed traces passed this quality control step and, as a matter of routine, we manually inspected randomly chosen subsets of valid and excluded traces to confirm appropriate categorisation and analysis.
All data sets passed this quality control.
All samples passed this quality control.
40 out of 57 samples (70%) passed this quality control.
In total, 331 (91.4 %) of the 362 primary cell samples passed this quality control procedure.
On average, 60% of the total number of sequenced 45 bp passed this quality read threshold.
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In total, 133 SNPs (92%) passed this quality-threshold filter and were used in the subsequent analysis.
The SNPs that do not pass this quality inspection can be discarded without affecting the results obtained on other SNPs.
After passing this quality control stage, the samples were hybridized onto the large Human Genome U133 Plus 2.0 Arrays (Affymetrix).
Samples which passed this initial quality control assurance step were then amplified one round, using an RNA Amplification Kit (Ambion) according to the manufacturer's instructions.
Samples which passed this initial quality control assurance step were then amplified one round, using an Illumina TotalPrep Kit (Ambion) to generate cDNA then cRNA according to the manufacturer's instructions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com