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Hence, this low passed filter transforms and smooths gradually the initial uneven Bouguer anomalies (Fig. 4) into smoothed anomalies highlighting regional crustal features.
This operation consists of the application of a low passed filter that attenuates short wavelengths while amplifying long wavelengths (Jacobsen 1987).
The 454 sequencing from 8 kb paired end libraries yielded 677,705 reads that passed filter wells with a 53.9% paired end ratio and a total chromosomal coverage of 29X.
This is also the case when comparing lane passed filter (PF) rates and the percentage of PF reads where the average base call quality is above Q30, for lanes with similar number of generated reads (Figure S1).
Passed filter wells for each genome was relatively homogeneous and represented from 20.1Mb for FR3713 to 28.5 Mb for FR743, which corresponds approximately to a fivefold recovery when considering a genome size of 4 Mb for B. pertussis.
Unsupervised hierarchical cluster analysis of the most differentially expressed genes (SD>1.5; 313 genes passed filter) of the entire cohort confirmed npCR samples to be enriched in the subtype with a basal-like phenotype (ER-negative, HER-2-negative, KRT5-positive) compared to the ER-negative/HER-2-positive-subtype (Figure 6b).
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Approximately 87.9% of clusters passed filtering.
10,980 promoter passed this filter.
177 genes passed this filter.
High pass filter.
BpFilter:band-pass filter.
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