Sequences passing cut-off value of 1e-10 were further analysed by BLASTx algorithm against Arabidopsis protein database, and those returning original Arabidopsis protein were listed.
A bidirectional filter with low pass cut off frequency of 5 Hz was used, and a bidirectional high pass filter with cut off frequency of 0.0159 Hz for step 1. High pass filter settings were variable for steps 2 4.
Functional data were spatially smoothed (full width half maximum = 5 mm) and temporal filtering (high pass cut off = 90 s) was also applied.
Preprocessing included 3D motion correction, slice acquisition time correction, normalization into MNI space (Montreal Neurological Institute), spatial smoothing (FWHM 12 mm), and temporal filtering (high pass cut off period 78 s, low pass kernel FWHM 4 s).
A linear model that accounted for first-order autocorrelations and low-frequency drifts (high pass cut off period 600 s) was used to estimate blood oxygen level-dependent signal amplitudes during each experimental condition for each participant.
Processing of the raw EMG signal was performed by converting it to RMS, band pass filtrered between 20 and 500 Hz, in order to subtract electromagnetic noise, and movement artefacts were filtered with a high pass cut off frequency of 20 Hz [ 25].
High-pass filtering (cut off 128s) was performed to remove low frequency artefacts.
Signals were band-pass filtered (cut off: 10 330 Hz) and digitally sampled at a frequency of 1000 Hz (full-wave rectification, fourth-order, zero-lag Butterworth, cut off: 100 Hz).
A high-pass frequency filter (cut off 120 sec) and corrections for auto-correlation between scans (AR1) were applied to the time series.
Only if you want to pass out -- you essentially cut off the circulation in your body.
