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The effect of these passages upon me was so electrifying that I can still remember sitting toward the back of the hall, surrounded by dark breathing bodies, barely able to see Mr Doctorow behind the podium on the stage, and yet alive with the idea of Freud and Jung emerging from that tunnel, and then with the image of the bicycle wheels of Houdini's plane spinning in the breeze.
Cryopreserved MSC were thawed and cultured up to five passages upon purchase from Lonza (Walkersville, MD, USA), prior to being utilized for this study.
Chondrocytes of P1 are generally considered nondedifferentiated [ 28] and authentic chondrocyte dedifferentiation is often developed after 4 passages upon 2D expansion, after which it is considered that the redifferentiation cannot be achieved in 3D culture without chondrogenic induction factors [ 4, 29].
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Cells were maintained and passaged upon reaching confluence by standard cell culture techniques.
MSCs were passaged upon reaching 50% confluency and cells from passage 2 4 were used for this study.
Human fibroblasts were isolated from fetal liver and grown in Isocove's modified Dulbecco's medium containing 10% FBS, and passaged upon confluence [1].
SFFs were passaged upon reaching 95% confluence with 0.25% trypsin-EDTA and infected with reprogramming virus between passages 3 and 5. 293T cells (ATCC, Manassas, VA, USA) were maintained in the same medium.
Cells were passaged upon reaching confluence.
Cells were passaged upon 75-80% confluence and counted.
Generally, cells were passaged upon reaching 75% confluence.
These cells were passaged upon reaching confluence over a 6-month period.
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