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And what a wonderful exploration of a child's way of tunneling into corners, through passages, up stairways.
Practicing Sequencing Activities Sequencing activities — cutting passages up and having students put them back in the correct order — is a common language-learning task in the E.S.L. classroom.
She had a stuffy nose, so I started the process of trying to clear her nasal passages up and tried to put the saline in her nose, and it was clear she didn't like this, and I thought, "I need to get the saline in there somehow.
Late-EPCs proliferated slowly during the first weeks, but then proliferated more rapidly and could be serially expanded in vitro through multiple passages, up to 7 10 weeks, when they finally stopped growing and entered senescence.
Saturation binding repeated at intervals throughout the experimental period verified that the levels observed initially were maintained through multiple passages (up to 30), demonstrating the stable nature of α7-nAChR expression by this cell line.
For all experiments ASC passages up to passage 2 were used.
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This interval of time (capture time) ranges from less than one day (a single passage), up to 500 days, or even more.
We fully-sequenced the genome of a historical prototype strain (L2/434/Bu) and a clinical isolate (E/CS88), before and after one-year of serial in vitro passaging (up to 3500 bacterial generations).
The inserting gene could be expressed consistently when the recombinant virus was passaged up to twenty times in cell cultures as determined by immunofluorescence assay (IFA) and genomic sequencing.
The cells could be passaged up to 65 times, beyond which no attempts were undertaken.
The cells were tripsinized, split 1∶4 and passaged up to three times.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com